Part:BBa_I716000:Design

wbbL

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

Bbrick ORF wbbL
[pcr] wbbL-F/R on pJ23006-Bca9106        (826bp, BglII/XhoI)
[sub] into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
[prod] pBca9145-wbbL (I716000)

---
AY001.wbbL-F     ccaagAGATCTatggtatatataataatcg
AY002.wbbL-R     gctagCTCGAGttaGGATCCttacgggtgaaaaactgatg

Source

pJ23006-Bca9106